6 September

Introduction and Developmental Anatomy

Readings

• Chapter 1

Technique

Fate mapping: dye injection

8 September

Developmental Genetics

Readings

• Chapter 2, pp 31 - 47

Technique

transgenesis

13 September

Developmental Genetics

Readings

• Chapter 2, pp 48 - 68

Technique

Animal cloning

Assignment

A chance to remember Introductory Biology: PDF DOC

15 September

Cell-Cell Communication

Readings

• Chapter 3, 69 - 84

Technique

in situ hybridization

20 September

Cell-Cell Communication 2

Readings

• Chapter 3, 84 - 107; 109-119

Assignment

Short (100 to 400 words) compare/contrast of Specification, Differentiation, and Commitment.

22 September

Early Development of Amphibians. Reading a scientific paper

Readings

• Chapter 7, 241-251
• Reading a Scientific Paper.

Technique

Homologous recombination

27 September

Paraxial mesoderm: mouse mesoderm and Tbx 6

Readings

Devoto

• Naiche, et al. (2005). T-box genes in vertebrate development. Ann. Rev. Genet. 39:219-239.
• Chapman and Papaioannou (1998). Three neural tubes in mouse embryos with mutations in the T-box gene Tbx6. Nature 391: 695-697.
• See Textbook Website 11.1

Assignment (email due 8PM the day before)

Turn in two questions related to the Chapman article.

29 September

NOON, 107 Shanklin: Deborah Chapman Seminar

29 September

Discussion of Chapman seminar

Readings

None.

DUE

Experimental Techniques Handbook (PART I)

4 October

Early Development in snails

Laidley and Kim Presentation

Readings

Everton Laidley, Dong Kim 

• Chapter 5, pp 159 - 164, and 178 - 185
• Patel NH. 2009 Developmental biology: Asymmetry with a twist. Nature 462:727-8.
• Kuroda R, Endo B, Abe M, Shimizu M. (2009) Chiral blastomere arrangement dictates zygotic left-right asymmetry pathway in snails. Nature 462:790-4.
• See Textbook Website 5.2

Assignment (email due 8PM the day before)

Email me two questions regarding the Kuroda article

6 October

Early Development in Tunicates and c. elegans

Readings

• Chapter 5, pp 187 - 202    

11 October

Early development in Drosophila, genetic screens

Readings

• Chapter 6, pp 203 - 217

Technique

Genetic Screen in Drosophila

13 October

Genetics of Axis Specification in Drosophila: Anterior-Posterior and dorsal ventral

Kozloff and Petzoldt presentation

Readings

Jill Kozloff; Jane Petzoldt

• Chapter 6, pp 218 - 239
• Ephrussi A, St Johnston D. 2004 Seeing is believing: the bicoid morphogen gradient matures. Cell. Jan 23;116(2):143-52.
• Driever W, Thoma G, Nüsslein-Volhard C. Nature. 1989 Determination of spatial domains of zygotic gene expression in the Drosophila embryo by the affinity of binding sites for the bicoid morphogen. Aug 3;340(6232):363-7.

18 October

Exam I

20 October

Axis formation, finish flies and begin frogs

Readings

·   Chapter 7, pp 252 - 271

FALL BREAK!!!

27 October

Axis formation, frogs: Fish. Testing a gradient model

Firer and Anthony Presentation

Readings

Mikhail Firer; Nicholas Anthony

• Chapter 7, pp 272 - 286
• Vincent S, Perrimon N (2001) Fishing for morphogens. Nature 411: 535-6
• Chen and Shier (2001). The zebrafish Nodal signal Squint functions as a morphogen. Nature 411: 607-10

Assignment (email due 8PM the day before)

 Turn in two questions related to the Chen article.

1 November

NO CLASS--POWER OUT

3 November

Axis formation frogs, Chick axis..

McLaren and Buehler Presentation

·   Chapter 8, pp 311 - 322

·   Burke, A. C., C. E. Nelson, B. A. Morgan and C. Tabin. 1995. Hox genes and the evolution of vertebrate axial morphology. Development 121: 333–346.

Technique

Genetic Screens in zebrafish

8 November

Neural Crest and Placodes

Readings

·       Chapter 9, pp 333 - 341 and pp 359 - 372

10 November

Paraxial mesoderm: Segmentation and myogenesis

Keogh and Day Presentation

Mummini and Feng Presentation

Readings

Sinead Keogh; Staphanie Day

Swetha Mummini; Victoria Feng

·       Chapter 11, pp 413 – 428

·      Palmeirim I, Henrique D, Ish-Horowicz D, Pourquie O. (1997). Avian hairy gene expression identifies a molecular clock linked to vertebrate segmentation and somitogenesis. Cell 91:639-48.

·       Chapter 10, pp 373 - 392

·       Tian NM, Price DJ. 2005 Why cavefish are blind. Bioessays. 27:235-8.

·       Yamamoto Y, Byerly MS, Jackman WR, Jeffery WR. 2009 Pleiotropic functions of embryonic sonic hedgehog expression link jaw and taste bud amplification with eye loss during cavefish evolution. Dev Biol. 330:200-11.

15 November

Segmentation and Myogenesis in zebrafish

Haydar and Choe Presentation

·       Stellabotte F, and Devoto SH (2007). The teleost dermomyotome.  Dev Dyn 236:2432-2443.

·       Windner SE, et al. (submitted).  Tbx6 inhibition of primary myogenesis is required for dermomyotome development.

 Assignment (email due 8PM the day before)

 Turn in two questions related to the Windner article.

17 November

Limb Development

Lee and Byer Presentation

Tucker and Bernardo Presentation

Readings

Timothy Lee;Lennox Byer

·      Chapter 13, Limb development

·      Riddle, R. D., R. L. Johnson, E. Laufer and C. Tabin. 1993. Sonic hedgehog mediates the polarizing activity of the ZPA. Cell 75: 1401–1416.

Readings

Rebecca Tucker; Melissa Bernardo 

·       Ohuchi H, Takeuchi J, Yoshioka H, Ishimaru Y, Ogura K, Takahashi N, Ogura T, Noji S. 1998. Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4. Development. 125: 51-60.

22 November

Post-embryonic development: Insect metamorphosis and regeneration

Readings

·      Chapter 15, pp 550 - 555

 Assignment

Turn in outline of your perspective essay.

Thanksgiving Break

29 November

Developmental Plasticity

MacKenzie, Jefferson, McGinnis Presentation

Readings

Ethan MacKenzie; Jefferson A.; Chris McGinnis

·       Chapter 18, pp 659-667

·       Weaver IC. 2007. Epigenetic programming by maternal behavior and pharmacological intervention. Nature versus nurture: let's call the whole thing off.  Epigenetics. Jan-Mar;2(1):22-8. Epub 2007 Jan 15. Review.

·       Weaver IC, Cervoni N, Champagne FA, D'Alessio AC, Sharma S, Seckl JR, Dymov S, Szyf M, Meaney MJ. 2004. Epigenetic programming by maternal behavior.  Nat Neurosci. 8:847-54.

1 December

Medical Aspects: Developmental Therapies

Hughes and Bussani Presentation

Readings

Kevin Hughes

Jake Bussani

·       Chapter 17, pp 648 - 657

·       Hanna J, Wernig M, Markoulaki S, Sun CW, Meissner A, Cassady JP, Beard C, Brambrink T, Wu LC, Townes TM, Jaenisch R. 2007 Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science. Dec 21;318(5858):1920-3.

Assignment (email due 8PM the day before)

Turn in two questions related to the Hanna article

6 December

Developmental Mechanisms of Evolutionary Change

Readings

·      Chapter 19

 DUE

PERSPECTIVE ESSAY

8 December

Genetic Basis for Evolutionary Change

Leebron Presentation

Readings

Isabelle Gauthier; Cade Leebron 

·       Shubin NH, Dahn RD. (2004). Evolutionary biology: lost and found. Nature. 428:703-4.

·       Shapiro MD, Marks ME, Peichel CL, Blackman BK, Nereng KS, Jonsson B, Schluter D, Kingsley DM. (2004). Genetic and developmental basis of evolutionary pelvic reduction in threespine sticklebacks. Nature. 428:717-23.

Assignment (email due 8PM the day before)

Turn in two questions related to the Shapiro article

13 Dec.

Exam 2 PRACTICE EXAM

Practice Exam KEY

There will be lots of practice questions